Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images
Michael Chiang, Sam Hallman, Amanda Cinquin, Nabora Mochel, Adrian Paz, Shimako Kawauchi, Anne Calof, Ken Cho, Charless Fowlkes, Oliver Cinquin
icon Analysis of single cells in their native environment is a powerful method to address key questions in developmental systems biology. Confocal microscopy imaging of intact tissues, followed by automatic image segmentation, provides a means to conduct cytometric studies while at the same time preserving crucial information about the spatial organization of the tissue and morphological features of the cells. This technique is rapidly evolving but is still not in widespread use among research groups that do not specialize in technique development, perhaps in part for lack of tools that automate repetitive tasks while allowing experts to make the best use of their time in injecting their domain-specific knowledge. Here we focus on a well-established stem cell model system, the C. elegans gonad, as well as on two other model systems widely used to study cell fate specification and morphogenesis: the pre-implantation mouse embryo and the developing mouse olfactory epithelium. We report a pipeline that integrates machine-learning-based cell detection, fast human-in-the-loop curation of these detections, and running of active contours seeded from detections to segment cells. The procedure can be bootstrapped by a small number of manual detections, and outperforms alternative pieces of software we benchmarked on C. elegans gonad datasets. Using cell segmentations to quantify fluorescence contents, we report previously-uncharacterized cell behaviors in the model systems we used. We further show how cell morphological features can be used to identify cell cycle phase; this provides a basis for future tools that will streamline cell cycle experiments by minimizing the need for exogenous cell cycle phase labels.

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Michael Chiang, Sam Hallman, Amanda Cinquin, Nabora Reyes de Mochel, Adrian Paz, Shimako Kawauchi, Anne Calof, Ken Cho, Charless Fowlkes, and Oliver Cinquin. Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images. BMC Bioinformatics, 2015.

BibTeX Reference

@article{ChiangHCRPKCCFC_BMCB_2015,
    AUTHOR = "Chiang, Michael and Hallman, Sam and Cinquin, Amanda and de Mochel, Nabora Reyes and Paz, Adrian and Kawauchi, Shimako and Calof, Anne and Cho, Ken and Fowlkes, Charless and Cinquin, Oliver",
    TITLE = "Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images",
    JOURNAL = "BMC Bioinformatics",
    YEAR = "2015",
    VOLUME = "16:397",
    TAG = "biological_images"
}