Analysis of Gap Gene Reguation in a 3D Organism-Scale Model of the Drosophila melanogaster Embryo
James Hengenius, Michael Gribskov, Ann Rundell, Charless Fowlkes, David Umulis
icon The axial bodyplan of Drosophila melanogaster is determined during a process called morphogenesis. Shortly after fertilization, maternal bicoid mRNA is translated into Bicoid (Bcd). This protein establishes a spatially graded morphogen distribution along the anterior-posterior (AP) axis of the embryo. Bcd initiates AP axis determination by triggering expression of gap genes that subsequently regulate each other's expression to form a precisely controlled spatial distribution of gene products. Reaction-diffusion models of gap gene expression on a 1D domain have previously been used to infer complex genetic regulatory network (GRN) interactions by optimizing model parameters with respect to 1D gap gene expression data. Here we construct a finite element reaction-diffusion model with a realistic 3D geometry fit to full 3D gap gene expression data. Though gap gene products exhibit dorsal-ventral asymmetries, we discover that previously inferred gap GRNs yield qualitatively correct AP distributions on the 3D domain only when DV-symmetric initial conditions are employed. Model patterning loses qualitative agreement with experimental data when we incorporate a realistic DV-asymmetric distribution of Bcd. Further, we find that geometry alone is insufficient to account for DV-asymmetries in the final gap gene distribution. Additional GRN optimization confirms that the 3D model remains sensitive to GRN parameter perturbations. Finally, we find that incorporation of 3D data in simulation and optimization does not constrain the search space or improve optimization results.

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Text Reference

James Hengenius, Michael Gribskov, Ann Rundell, Charless Fowlkes, and David Umulis. Analysis of gap gene reguation in a 3d organism-scale model of the drosophila melanogaster embryo. PLoS ONE, 6:e26797, 2011.

BibTeX Reference

    AUTHOR = "Hengenius, James and Gribskov, Michael and Rundell, Ann and Fowlkes, Charless and Umulis, David",
    TITLE = "Analysis of Gap Gene Reguation in a 3D Organism-Scale Model of the Drosophila melanogaster Embryo",
    Journal = "PLoS ONE",
    Year = "2011",
    Volume = "6",
    Issue = "11",
    pages = "e26797",
    TAG = "biological_images"